Set up an Analysis
Set up analyses on the Projects page using the Set up Analysis wizard.
The following elements must be in place:
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MS data files have been uploaded to PAS. For information, see Upload MS data files.
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Plates have been created. For information, see Add a project.
To set up an analysis:
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On the Projects page, select a project and then select Set up Analysis.
TheSet up Analysis wizard opens.
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Complete the fields on the wizard:
- Analysis name
- Assign a clear, descriptive name for your analysis for easy identification. (Required)
- Analysis description
- Provide a brief overview of the analysis purpose and key features.
- Analysis notes
- Add additional notes for the analysis.
- Analysis protocol
- Select the protocol to use for the analysis. Select from the protocol types and use the dropdown to select the specific one. After you select a protocol, the Analysis Preotocol Details pane populates with details about the selected protocol.
- Peptide calibrant
- Specify if Seer’s Peptide Calibration Kit was used during the preparation of these samples. (Required for select analysis protocols)
- Folder
- Select a folder where the analysis details will be saved. (Folders are recommended as a best practice to help organize data.)
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Select Continue to save your settings.
The wizard advances to view the Samples Selection page where you can select the samples for your analysis. Like other tables in PAS, you can show and hide columns and data in columns can be sorted and filtered. You can also determine how many samples are shown in each page of the table. For information on working with tables, see PAS tables.
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On the Samples Selection (Step 2 of 3) page, select samples for the analysis. This page has two tabs:
- Available Samples
- Includes all samples in the project that are available. A number identifies next to the tab name indicates how many samples are on the tab. Samples you select are removed from this tab and appear on the Selected for Analysis tab.
- Selected for Analysis
- Includes the samples selected for the analysis. Removing a sample moves it back to the Available Samples tab.
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To include samples:
On the Available Samples tab, select the checkbox next to a sample to include it in the analysis. (You can select multiple checkboxes.)
OR
Select the checkbox in the table head row to select all samples on the page.Your plate may contain more samples than are visible in one page of the table.
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Select Add to Analysis to add samples to the analysis.
Selected samples are moved to the Selected for Analysis tab.
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You can remove samples by selecting the checkbox in front of a sample and selecting Remove from Analysis. Removed samples are moved to the Available Samples tab.
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Select Continue to save your settings.
The Review Analysis Setup page appears with a summary of the samples, controls, and plates that are included in the analysis.
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Select Start Analysis to begin the analysis.